Okie doke. When you first call the scanner from your imaging program, you will likely see this face of the driver. We don't want this Full Auto Mode. Dropping down the menu, you will see two choices. Click on Home mode.

Here it is, but we don't want this one either. Drop the menu and select Professional mode.

Ahhh... there we are!

Load your film trays, pull the cover on the tranparency bulb. Sort through the menus to change document type from reflective to transparency, film type, image type (color depth, etc), then click the preview button.

You may have to wait a few for the scanner to warm up. Once we have our previewing done, select what you wish to scan by lassoing it.

Once you have more-or-less what you wish to zoom in to, click the zoom button. Once zoomed, you can refine your crop.

Find the histogram tool button and click it.

I generally pull my lower sliders to their extreme left/right movements, then make final adjustments accordingly on the upper levels sliders. I go to the threshhold on black/white points and eyeball it for contrast. I will make further adjustments once I am scanned.

Here is the zoom for final inspection (checking crop, etc) before executing the scan. I scan 35mm film at 1200dpi, 120film at 600. Prints have been well within expectations at 8x10. You can scan deeper if desired, but that's my limit. Do not, I never adjust the scale pre-scan. I do all of that resizing post-scan.

Good luck!